The polyamine spermidine is a ubiquitous molecule linked to multiple health benefits. It exists in free and bound (e.g., to DNA/RNA) forms, but both the subcellular and intercellular distribution is poorly understood. Free spermidine partly acts via the hypusination of eIF5A, but the relationship between hypusinated eIF5A and different metabolic pathays has not been addressed. Overall, it remains unclear whether aging or nutritional cues affect hyusination differently. […]
We hypothesize that metabolic pathways control eIF5A hypusination and other downstream effects. Our objectives are to 1 explore...